The Hardikar
Laboratory
Anandwardhan A.
Hardikar, PhD, received MSc in Zoology (Genetics) and a PhD in Zoology from the
Research in the
laboratory is focused on understanding islet biology and development of
insulin-producing cells. We work with
cadaveric human pancreatic islets as well as biliary duct and gallbladder-derived
cells to gather information that would help us understand development of
insulin-producing cells. Our previous
studies using next generation sequencing of developing human pancreas using the
SOLiD platform have provided insight to understanding the role of ncRNAs
(specifically microRNAs) in development and differentiation of
insulin-producing cells. Present
research projects involve applying this information to differentiation of human
pancreatic progenitor cells. In addition
to these studies, other projects in the lab are focused on understanding the
epigenetic modifications in insulin-producing cells in a unique model of
multigeneration undernutrition. These
studies involve understanding the influence of diet, micronutrients, intrautetine
programming and gut microbiota in development of central adiposity, insulin
resistance and type 2 diabetes.
Contents 
Laboratory
group / PhD students
Work Information
Diabetes and Pancreas Biology
Group,
The O’Brien Institute, St.
Vincent’s Hospital and the
Off: (03) 9288 4018
E-mail: anandh@unimelb.edu.au
(For area map, click here)
nccs
Stem Cells and Diabetes Section,
Pune 411007,
Lab: +91 20 2570 8150
Fax: +91 20 2569 2259
E-mail: anand@isletbiology.com
(For area map, click here)
Research
articles:
·
Satoor SN,
·
Vaithilingam V, Quayum N,
Joglekar MV, Jensen J, Hardikar AA, Oberholzer J, Guillemin GJ, Tuch BE (2011) Effect of alginate
encapsulation on the cellular transcriptome of human islets, Accepted for
publication in Biomaterials
·
Joglekar MV and Hardikar AA
(2011) Isolation, characterization and expansion of islet-derived progenitor
cells. Accepted for publication in Methods in Molecular Biology
·
Kumar U,
·
Forster N, Penington A,
Hardikar AA, Palmer J, Hussey A, Taj J, Morrison W, Feeney S (2011) A
prevascularised tissue engineering chamber supports growth and function of
islets and progenitor cells in diabetic mice. Islets (In press)
·
Parekh VS, Umrani MR and
Hardikar AA (2011) Taurine supports preservation of pro-endocrine cell types in
human umbilical cord blood-derived mononuclear cells during cryostorage. Transplant Proc (In
press)
·
Phadnis SM, Joglekar MV,
Dalvi1 MP, Muthyala S, Nair PD, Ghaskadbi SM, Bhonde RR and Hardikar AA (2010)
Human bone marrow-derived mesenchymal cells differentiate and mature into
endocrine pancreatic lineage in vivo. Cytotherapy (in press) Cover story
·
Ansarullah, Jayaraman S,
Hardikar AA and Ramachandran AV (2010) Influence of Oreocnide integrifolia
(Gaud.) Miq on IRS-1, Akt and Glut-4 in Fat-Fed C57BL/6J Type 2 Diabetes Mouse
Model. Evid-Based
Complement Alternat Med (In press).
·
Joglekar MV, Wei CJ and
Hardikar AA (2010) Quantitative estimation of multiple microRNAs and mRNAs from
a single cell. Cold Spring Harb. Protocols Cover story
·
Khandwekar AP, Patil DP,
Hardikar AA, Shouche YS, Doble M. (2010) In vivo modulation of foreign body
response on polyurethane by surface entrapment technique. J
Biomed Mater Res A. (In press)
·
Wu Y, Li J, Saleem S, Yee S,
Hardikar AA and Wang R (2010) C-Kit and stem cell factor regulate PANC-1 cell
differentiation to insulin- and glucagon-producing cells. Lab
Investigation (In press)
·
Sasikala M, Swapna G, Pavan K,
Rao GV, Hardikar AA and Reddy DN (2010) Reduced expression of pdx-1 correlates
with decreased beta cell function in chronic pancreatitis. Pancreas (In press)
·
Lee J, Jo J, Hardikar AA, Periwal V and
Rane SG (2010) Cdk4 regulates recruitment of
quiescent β-cells and ductal epithelial progenitors to reconstitute
β-cell mass PLoS
ONE
13;5(1):e8653
·
Phadnis
SM, Ghaskadbi SM, Hardikar AA, Bhonde RR (2009) Mesenchymal stem cells derived
from bone marrow of diabetic patients portrait unique markers influenced by the
diabetic microenvironment. Rev
Diabet Stud. 6(4):260-70.
·
Ranjan
AK, Kumar U, Hardikar AA, Poddar P, Nair PD, Hardikar AA (2009) Human blood
vessel-derived endothelial progenitors for endothelialization of small diameter
vascular prosthesis. PLoS
ONE 4(11):e7718.PMID: 19890388
·
Joglekar MV, Patil D, Joglekar VM, Rao
GV, Reddy DN, Sasikala M, Shouche Y and Hardikar AA (2009) The
miR-30 Family microRNAs Confer Epithelial Phenotype to Human Pancreatic Cells. Islets 1(2): 137
- 147. Cover story
·
Parekh VS, Joglekar MV and Hardikar AA (2009)
Differentiation of human umbilical cord blood derived mononuclear cells to
endocrine pancreatic lineage. Differentiation
78(4):232-40. Cover story
·
Joglekar MV, Joglekar VM, Joglekar SV and Hardikar
AA (2009), Human fetal pancreatic insulin-producing cells proliferate in vitro.
J.
Endocrinology 201(1):27-36, Epub 2009 May 6 Cover story
·
Sahu S*, Joglekar MV*, Dumbre R, Phadnis SM, Tosh
D and Hardikar AA, (2009) Islet-like cell clusters occur
naturally in human gall bladder and are retained in diabetic conditions J Cell Mol
Med: 13(5):999-1000; *: equal first authors.
·
Joglekar
MV, Joglekar VM and Hardikar AA (2009) Expression of islet-specific microRNAs
during human pancreatic development. Gene
Exp Patterns; 9(2):
109-113. Epub 2008 Oct 17
·
Joglekar
MV, Parekh VS, Mehta S, Bhonde RR and Hardikar AA (2007) MicroRNA Profiling of
Developing and Regenerating Pancreas Reveal Post-transcriptional Regulation of
Neurogenin3. Developmental
Biology 311(2): 603-612
·
Phadnis
SM*, Joglekar MV*, Venkateshan V, Ghaskadbi SM, Hardikar AA, Bhonde RR. (2006)
Human umbilical cord blood serum promotes growth, proliferation, as well as
differentiation of human bone marrow-derived progenitor cells. In
Vitro Cell Dev Biol Anim. 42(10):283-6.
·
Gershengorn
MC, Geras-Raaka E, Hardikar AA, Raaka BM. (2005) Are
better islet cell precursors generated by epithelial-to-mesenchymal transition?
Cell
Cycle. 4:380-382.
·
Gershengorn
MC, Hardikar AA, Wei C, Geras-Raaka E, Marcus-Samuels B, Raaka BM (2004)
Epithelial-to-Mesenchymal Transition Generates Proliferative Human Islet
Precursor Cells. Science
306:2261-2264.
·
Hardikar
AA, Marcus-Samuels B, Geras-Raaka E, Raaka BM, Gershengorn MC (2003) Human
pancreatic precursor cells secrete FGF2 to stimulate clustering into
hormone-expressing islet-like cell aggregates. Proc
Natl Acad Sci U S A.
100(12): 7117-7122.
·
Hardikar
AA, Wang XY, Williams LJ, Kwok J, Wong R,
·
Merezek
S, Hardikar AA, Yajnik CS, Remacle C and Reusens B (2001) Intra-uterine protein
malnutrition and diabetes: the role of sulphur amino acids. J
Endocrinol; 171:299-308.
·
Hardikar
AA, Risbud MV, Remacle C, Reusens B, Hoet JJ, Bhonde RR. (2001) Islet
cryopreservation: improved recovery following taurine pretreatment. Cell
Transplant; 10:247-253.
·
Hardikar
AA, Nath BB. (2001) Chromosomal polymorphism is associated with nematode
parasitism in a natural population of a tropical midge. Chromosoma;
110: 58-64.
·
Risbud MV,
Hardikar AA, Bhat SV, Bhonde RR. (2000) pH-sensitive freeze-dried chitosan-polyvinyl pyrrolidone
hydrogels as controlled release system for antibiotic delivery. J
Control Release; 68: 23-30.
·
Hardikar
AA, Risbud MV, Bhonde RR. (2000) Improved
post-cryopreservation recovery following encapsulation of islets in
chitosan-alginate microcapsules. Transplant
Proc; 32: 824-5.
·
Risbud
M, Hardikar A, Bhonde R. (2000) Growth modulation of fibroblasts by
chitosan-polyvinyl pyrrolidone hydrogel: implications for wound management? J
Biosci ; 25: 25-31.
·
Risbud
M, Hardikar A, Bhonde R. (2000) Chitosan-polyvinyl pyrrolidone hydrogels as
candidate for islet immunoisolation: in vitro biocompatibility evaluation. Cell
Transplant; 9 : 25-31.
·
Hardikar
AA, Bhonde RR. (1999) Modulating experimental diabetes by treatment with
cytosolic extract from the regenerating pancreas. Diabetes
Res Clin Pract; 46: 203-11.
·
Hardikar
AA, Karandikar MS, Bhonde RR. (1999)
Effect of partial pancreatectomy on diabetic status in BALB/c mice. J
Endocrinol ; 162: 189-195.
·
Hardikar
AA, Risbud MV and Bhonde RR (1999) A simple microcapsule generator design for
islet encapsulation, J.
Biosci. 24 (3): 371 - 376.
Chapters
in books:
·
Joglekar MV and Hardikar AA (2010) Human pancreatic progenitors: implications for clinical
transplantation in Diabetes in “Human Fetal Tissue in Transplantation” eds. Dr
Niranjan Bhattacharya and Prof Phillip Stubblefield. Springer Verlag,
·
Joglekar MV,
·
Hardikar AA and Gershengorn MC (2003) Chapter 51:
‘Stem Cells’ in 3rd edition of ‘Diabetes Mellitus: A Fundamental and Clinical
Text’, edited by: Dr. Derek LeRoith, Simeon I. Taylor and Jerrold M. Olefsky.
·
Shukla R, Risbud MR, Hardikar AA, Bhonde RR, (2004)
Chitosan and chitin derivatives in islet immunoisolation and transplantation.
In “Chitin and Chitosan: Opportunities and Challenges” Chapter 15, p 211-222,
eds; Dutta PK
Invited
Reviews:
·
Joglekar MV, Parekh VS and Hardikar AA (2011)
microRNAs in endocrine pancreas development, differentiation and disease. In J Exp. Biol 49(6):401-8.
·
·
Williams MD, Mitchell GM and Hardikar AA, Stem
cells: Epigenetic basis of differentiation (2010) Open Stem Cells (in press)
·
Dalvi MP, Umrani MR, Joglekar MV, Hardikar AA (2009)
Human pancreatic islet progenitor cells demonstrate phenotypic plasticity in
vitro. J
Biosci. 34(4):523-8.
·
·
Kode JA, Mukherjee S,
Joglekar MV and Hardikar AA (2009) Mesenchymal Stem cells: Immunobiology and
role in immunomodulation and tissue regeneration. Cytotherapy
: 11(4):377-91
·
Joglekar
MV, Parekh VS and Hardikar AA (2007) New Pancreas from Old: Microregulators of
Pancreas Regeneration Trends
Endocrinol Metab 18(10):393-400.
Cover
citation.
·
Hardikar
AA, Lees JG,
·
Hardikar AA (2004) Pancreas regeneration: Generating
new pancreas from old. Trends
Endocrinol Metab. 15:198-203
Cover story
Editorial
/commentaries:
·
Joglekar MV and Hardikar AA
(2010) Epithelial-to-mesenchymal
transition in pancreatic islet beta cells. Cell Cycle (in press)
·
Hardikar AA and Tosh D
(2009) Commentary: β-cells in the lab. Nature India, doi 10:1038/nindia.2009.160
·
Sahu S, Tosh D and Hardikar
AA (2009) Commentary: New sources of beta-cells for treating diabetes. J.
Endocrinology: 202(1):13-6
·
Hardikar A, (2004) Role of
Incretins in Pancreas Growth and Development. J Pancreas 5(6):454-456.
Current Projects
The RAPID Study:
RNA-based Analysis for Prediction of Islet Death
Understanding
the role of ncRNAs in pancreatic development, differentiation and disease
Role
of micronutrients and gut microbiota in development of central adiposity and
diabetes
Differentiation
of human gallbladder-derived endocrine progenitor cells
Developmental
biology of ectopic pancreas using Pdx1-GFP / MIP-GFP mice
Epigenetic mechanisms regulating beta cell function
Diabetes
complications: Endothelial cell damage
and Proliferative Diabetic Retinopathy
Research facilities in the Hardikar
laboratory include a human cell culture facility, rodent cell culture facility,
human and mouse ES cell culture facility, real-time pcr (Applied Biosystems
7500 FAST, 7900 HT FAST block and TLDA systems), Calcium ratio imaging system
(Till Photonics), Axio Observer Z1 with Apotome imaging, PALM microbeam 4 laser
catapulting system and in vivo imaging facility. Other accessible central
facilities include proteomics and microarray core, flowcytometry core (BD FACS
Vantage, MoFlow, Aria, Caliburs, Canto II) and imaging facility (LSM 510, TIRF,
FCS, time-lapse microscopy), DNA sequencing facility and high throughput
sequencing (Applied Biosystems SOLiD).
Research in the Hardikar laboratory
has been funded by intramural funding from the National
Center for Cell Science and research project grants from the Department of Biotechnology, Government
of India and the UK-India Educational Research
Initiative (UKIERI) / British Council,
Research work at O’Brien Institute
is supported through generous funding from Microsurgery Foundation and The
Barbara Walker Fellowship.
Laboratory group / PhD students
The research group is based at the
O’Brien Institute,
For more information about existing
and past lab members from the Hardikar lab, please click
here
Research Collaborators:
Prof.
Thomas Kay and Helen Thomas, St. Vincent’s Institute,
Prof.
Alicia Jenkins, St. Vincent’s Hospital,
Dr.
Alex Hewitt, Center for Eye Research
Prof.
Shimon Efrat,
Dr. Paul Verma,
Monash Institute of Medical Research,
Prof. Ed
Stanley, Monash Immunology and Stem Cells Laboratories,
Dr. David Tosh,
Dr. Sanjeev
Galande, Indian Institute of Science and Education Research, Pune
Dr. DN Reddy, Dr. GV
Rao and Dr Sasikala Mitnala, Asian Institute of
Dr. Yogesh Shouche, Microbial
Dr.
Sushil G. Rane, National Institutes of Health,
Research Support:
Prof.
Len Harrison, WEHI,
Dr.
Dhan Thiruchelvam, St. Vincent’s Hospital,
Dr.
Vinay and Dr. Sheela Joglekar, Shree Seva Medical Foundation, Shirwal
Dr.
Medha Patankar, Patankar Nursing Home, Pune
The Hardikar
laboratory is located at The O’Brien Institute, St. Vincents’ Hospital campus
in Fitzroy,
Last revised: June 23, 2010