The Hardikar Laboratory

Anandwardhan A. Hardikar, PhD, received MSc in Zoology (Genetics) and a PhD in Zoology from the University of Pune.  After successful completion of his PhD work, carried out mainly at the National Center for Cell Science and partly at the WHO center, Catholic University of Louvain, Louvain-La-Neuve, Belgium, he continued training in the field of pancreas biology and diabetes at the University of Pennsylvania, School of Medicine, where he worked with Doris Stoffers.  He then went on to Sydney, Australia to work with Prof. Bernie Tuch at the University of New South Wales where he pursued his research interests in transplantation of insulin-producing surrogate b-cells.  After his research tenure in the laboratory of Marvin Gershengorn, Scientific Director, NIDDK, NIH, Bethesda, MD, he went on to join the National Center for Cell Science, Pune, India.  Presently, Dr. Hardikar leads the Diabetes and Pancreas Biology Section at O’Brien Institute, St. Vincent’s Hospital and the University of Melbourne, in Melbourne, Australia.

Research in the laboratory is focused on understanding islet biology and development of insulin-producing cells.  We work with cadaveric human pancreatic islets as well as biliary duct and gallbladder-derived cells to gather information that would help us understand development of insulin-producing cells.  Our previous studies using next generation sequencing of developing human pancreas using the SOLiD platform have provided insight to understanding the role of ncRNAs (specifically microRNAs) in development and differentiation of insulin-producing cells.  Present research projects involve applying this information to differentiation of human pancreatic progenitor cells.  In addition to these studies, other projects in the lab are focused on understanding the epigenetic modifications in insulin-producing cells in a unique model of multigeneration undernutrition.  These studies involve understanding the influence of diet, micronutrients, intrautetine programming and gut microbiota in development of central adiposity, insulin resistance and type 2 diabetes.     

 

Contents                                            

*      Contact information

*      Publications

*      Current projects

*      Laboratory group / PhD students

*      Collaborators

 

Work Information

                                       

     Diabetes and Pancreas Biology Group,

     The O’Brien Institute, St. Vincent’s Hospital and the University of Melbourne,

     42 Fitzroy Street, Melbourne VIC 3065

     Australia

      Off: (03) 9288 4018

      E-mail: anandh@unimelb.edu.au

(For area map, click here)

 

                                                  nccs

Stem Cells and Diabetes Section,

National Center for Cell Science, Ganeshkhind Road,
Pune 411007, India
Lab: +91 20 2570 8150 

Fax: +91 20 2569 2259

E-mail: anand@isletbiology.com

(For area map, click here)

 

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Research articles:

 

·         Joglekar MV, Wei CJ and Hardikar AA (2010) Quantitative estimation of multiple microRNAs and mRNAs from a single cell.  Cold Spring Harb. Protocols  Cover story

 

·         Ansarullah, Jayaraman S, Hardikar AA and Ramachandran AV (2010) Influence of Oreocnide integrifolia (Gaud.) Miq on IRS-1, Akt and Glut-4 in Fat-Fed C57BL/6J Type 2 Diabetes Mouse Model. Evid-Based Complement Alternat Med  (In press).

 

·         Khandwekar AP, Patil DP, Hardikar AA, Shouche YS, Doble M. (2010) In vivo modulation of foreign body response on polyurethane by surface entrapment technique. J Biomed Mater Res A. (In press)

 

·         Wu Y, Li J, Saleem S, Yee S, Hardikar AA and Wang R (2010)  C-Kit and stem cell factor regulate PANC-1 cell differentiation to insulin- and glucagon-producing cells.  Lab Investigation  (In press)

 

·        Sasikala M, Swapna G, Pavan K, Rao GV, Hardikar AA and Reddy DN (2010) Reduced expression of pdx-1 correlates with decreased beta cell function in chronic pancreatitis.  Pancreas (In press)

 

·        Lee J, Jo J, Hardikar AA, Periwal V and Rane SG (2010) Cdk4 regulates recruitment of quiescent β-cells and ductal epithelial progenitors to reconstitute β-cell mass PLoS ONE  13;5(1):e8653

 

·        Phadnis SM, Ghaskadbi SM, Hardikar AA, Bhonde RR (2009) Mesenchymal stem cells derived from bone marrow of diabetic patients portrait unique markers influenced by the diabetic microenvironment. Rev Diabet Stud. 6(4):260-70.

 

·        Ranjan AK, Kumar U, Hardikar AA, Poddar P, Nair PD, Hardikar AA (2009) Human blood vessel-derived endothelial progenitors for endothelialization of small diameter vascular prosthesis. PLoS ONE 4(11):e7718.PMID: 19890388

 

·        Joglekar MV, Patil D, Joglekar VM, Rao GV, Reddy DN, Sasikala M, Shouche Y and Hardikar AA (2009) The miR-30 Family microRNAs Confer Epithelial Phenotype to Human Pancreatic Cells.  Islets  1(2): 137 - 147. Cover story

 

·        Parekh VS, Joglekar MV and Hardikar AA (2009) Differentiation of human umbilical cord blood derived mononuclear cells to endocrine pancreatic lineage.  Differentiation 78(4):232-40. Cover story

 

·        Joglekar MV, Joglekar VM, Joglekar SV and Hardikar AA (2009), Human fetal pancreatic insulin-producing cells proliferate in vitro.  J. Endocrinology  201(1):27-36, Epub 2009 May 6 Cover story

 

·        Sahu S*, Joglekar MV*, Dumbre R, Phadnis SM, Tosh D and Hardikar AA, (2009) Islet-like cell clusters occur naturally in human gall bladder and are retained in diabetic conditions J Cell Mol Med: 13(5):999-1000; *: equal first authors.

 

·        Joglekar MV, Joglekar VM and Hardikar AA (2009) Expression of islet-specific microRNAs during human pancreatic development.  Gene Exp Patterns; 9(2): 109-113. Epub 2008 Oct 17

 

·        Joglekar MV, Parekh VS, Mehta S, Bhonde RR and Hardikar AA (2007) MicroRNA Profiling of Developing and Regenerating Pancreas Reveal Post-transcriptional Regulation of Neurogenin3. Developmental Biology  311(2): 603-612

 

·        Phadnis SM*, Joglekar MV*, Venkateshan V, Ghaskadbi SM, Hardikar AA, Bhonde RR. (2006) Human umbilical cord blood serum promotes growth, proliferation, as well as differentiation of human bone marrow-derived progenitor cells. In Vitro Cell Dev Biol Anim. 42(10):283-6.

 

·        Gershengorn MC, Geras-Raaka E, Hardikar AA, Raaka BM. (2005) Are better islet cell precursors generated by epithelial-to-mesenchymal transition? Cell Cycle. 4:380-382.

 

·        Gershengorn MC, Hardikar AA, Wei C, Geras-Raaka E, Marcus-Samuels B, Raaka BM (2004) Epithelial-to-Mesenchymal Transition Generates Proliferative Human Islet Precursor Cells.  Science 306:2261-2264.

 

·        Hardikar AA, Marcus-Samuels B, Geras-Raaka E, Raaka BM, Gershengorn MC (2003) Human pancreatic precursor cells secrete FGF2 to stimulate clustering into hormone-expressing islet-like cell aggregates. Proc Natl Acad Sci U S A. 100(12): 7117-7122. 

 

·        Hardikar AA, Wang XY, Williams LJ, Kwok J, Wong R, Yao M, Tuch BE. (2002) Functional maturation of fetal porcine beta-cells by glucagon-like peptide 1 and cholecystokinin. Endocrinology; 143:3505-3514.

 

·        Merezek S, Hardikar AA, Yajnik CS, Remacle C and Reusens B (2001) Intra-uterine protein malnutrition and diabetes: the role of sulphur amino acids. J Endocrinol; 171:299-308.

 

·        Hardikar AA, Risbud MV, Remacle C, Reusens B, Hoet JJ, Bhonde RR. (2001) Islet cryopreservation: improved recovery following taurine pretreatment. Cell Transplant; 10:247-253.

 

·        Hardikar AA, Nath BB. (2001) Chromosomal polymorphism is associated with nematode parasitism in a natural population of a tropical midge. Chromosoma; 110: 58-64.

 

·        Risbud MV, Hardikar AA, Bhat SV, Bhonde RR. (2000) pH-sensitive freeze-dried chitosan-polyvinyl pyrrolidone hydrogels as controlled release system for antibiotic delivery. J Control Release; 68: 23-30.

 

·        Hardikar AA, Risbud MV, Bhonde RR. (2000) Improved post-cryopreservation recovery following encapsulation of islets in chitosan-alginate microcapsules. Transplant Proc; 32: 824-5.

 

·        Risbud M, Hardikar A, Bhonde R. (2000) Growth modulation of fibroblasts by chitosan-polyvinyl pyrrolidone hydrogel: implications for wound management? J Biosci ; 25: 25-31.

 

·        Risbud M, Hardikar A, Bhonde R. (2000) Chitosan-polyvinyl pyrrolidone hydrogels as candidate for islet immunoisolation: in vitro biocompatibility evaluation.  Cell Transplant; 9 : 25-31.

 

·        Hardikar AA, Bhonde RR. (1999) Modulating experimental diabetes by treatment with cytosolic extract from the regenerating pancreas. Diabetes Res Clin Pract; 46: 203-11.

 

·        Hardikar AA, Karandikar MS, Bhonde RR. (1999) Effect of partial pancreatectomy on diabetic status in BALB/c mice. J Endocrinol ; 162: 189-195.

 

·        Hardikar AA, Risbud MV and Bhonde RR (1999) A simple microcapsule generator design for islet encapsulation, J. Biosci. 24 (3): 371 - 376. 

 

Chapters in books:

 

·        Joglekar MV, Ranjan AK and Hardikar AA (2009) Pancreas development and regeneration.  “In Stem cells: Organogenesis and Cancer” Editor: Dr. SR Singh, Book Editor & Managing Editor: Research Signpost/ Transworld Research Network press (http://www.ressign.com/).  Frontiers in Bioscience, NIH, USA

 

·        Hardikar AA and Gershengorn MC (2003) Chapter 51: ‘Stem Cells’ in 3rd edition of ‘Diabetes Mellitus: A Fundamental and Clinical Text’, edited by: Dr. Derek LeRoith, Simeon I. Taylor and Jerrold M. Olefsky.

 

·        Shukla R, Risbud MR, Hardikar AA, Bhonde RR, (2004) Chitosan and chitin derivatives in islet immunoisolation and transplantation.  In “Chitin and Chitosan: Opportunities and Challenges” Chapter 15, p 211-222, eds; Dutta PK

 

Invited Reviews:

 

·        Dalvi MP, Umrani MR, Joglekar MV, Hardikar AA (2009) Human pancreatic islet progenitor cells demonstrate phenotypic plasticity in vitro. J Biosci. 34(4):523-8.

 

·        Ranjan AK, Joglekar MV and Hardikar AA (2009) Endothelial cells in pancreatic islet development and function Islets 1(1); 2 – 9

 

·        Kode JA, Mukherjee S, Joglekar MV and Hardikar AA (2009) Mesenchymal Stem cells: Immunobiology and role in immunomodulation and tissue regeneration. Cytotherapy : 11(4):377-91

 

·        Joglekar MV, Parekh VS and Hardikar AA (2007) New Pancreas from Old: Microregulators of Pancreas Regeneration Trends Endocrinol Metab  18(10):393-400. Cover citation.

 

·        Hardikar AA, Lees JG, Sidhu KS, Colvin E and Tuch BE; Stem-Cell Therapy for Diabetes Cure: How Close Are We? (2006) Current Stem Cell Research and Therapy: 1:425-436

 

·        Hardikar AA (2004) Pancreas regeneration: Generating new pancreas from old. Trends Endocrinol Metab. 15:198-203 Cover story

 

Editorial /commentaries:

 

·        Hardikar AA and Tosh D (2009) Commentary: β-cells in the lab. Nature India, doi 10:1038/nindia.2009.160

 

·        Sahu S, Tosh D and Hardikar AA (2009) Commentary: New sources of beta-cells for treating diabetes. J. Endocrinology: 202(1):13-6

 

·        Hardikar A, (2004) Role of Incretins in Pancreas Growth and Development.  J Pancreas 5(6):454-456.

 

 

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Current Projects

 

*      Understanding the role of ncRNAs in directed differentiation of human pancreatic islet-derived progenitor cells

*      Differentiation of human gallbladder-derived endocrine progenitor cells

*      Developmental biology of ectopic pancreas using Pdx1-GFP / Pdx1-rosa mice

*      Epigenetic mechanisms regulating beta cell function

*      Endothelial cell dysfunction in diabetes

*      Role of micronutrients and gut microbiota in development of central adiposity and diabetes

 

 

Research facilities in the Hardikar laboratory include a human cell culture facility, rodent cell culture facility, human and mouse ES cell culture facility, real-time pcr (Applied Biosystems 7500 FAST, 7900 HT FAST block and TLDA systems), Calcium ratio imaging system (Till Photonics), Axio Observer Z1 with Apotome imaging, PALM microbeam 4 laser catapulting system and in vivo imaging facility.  Other accessible central facilities include proteomics and microarray core, flowcytometry core (BD FACS Vantage, MoFlow, Aria, Caliburs, Canto II) and imaging facility (LSM 510, TIRF, FCS, time-lapse microscopy), DNA sequencing facility and high throughput sequencing (Applied Biosystems SOLiD).    

 

Research in the Hardikar laboratory has been funded by intramural funding from the National Center for Cell Science and research project grants from the Department of Biotechnology, Government of India and the UK-India Educational Research Initiative (UKIERI) / British Council, UK.

Research work at O’Brien Institute is supported through generous funding from Microsurgery Foundation and The Barbara Walker Fellowship.    

 

 

                                

 

 

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Laboratory group / PhD students

 

The research group is based at the O’Brien Institute, Melbourne, Australia.  Potential students who would be interested in research in my laboratory can e-mail me their CV along with names and contact information of 3 referees.  PhD students at the O’Brien Institute are enrolled for their degree at The University of Melbourne, Melbourne, Australia.  International students can access fellowship possibilities for PhD programs by visiting the University of Melbourne “Future students” website. 

For more information about existing and past lab members from the Hardikar lab, please click here 

 

 

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Research Collaborators:

 

*      Prof. Thomas Kay and Helen Thomas, St. Vincent’s Institute, Melbourne, Australia

*      Prof. Ed Stanley, Monash Immunology and Stem Cells Laboratories, Clayton, Australia

*      Prof. Alicia Jenkins, St. Vincent’s Hospital, Melbourne, Australia

*      Dr. David Tosh, University of Bath, UK

*      Dr. Sanjeev Galande, Indian Institute of Science and Education Research, Pune

*      Dr. DN Reddy, Dr. GV Rao and Dr Sasikala Mitnala, Asian Institute of Gastroenterology, India

*      Dr. Yogesh Shouche, Microbial Containment Center, National Center for Cell Science, Pune, India

*      Dr. Sushil G. Rane, National Institutes of Health, Bethesda, USA

 

 

Research Support:

 

*      Dr. Dhan Thiruchelvam, St. Vincent’s Hospital, Melbourne.

*      Dr. Vinay and Dr. Sheela Joglekar, Shree Seva Medical Foundation, Shirwal

*      Dr. Medha Patankar, Patankar Nursing Home, Pune

 

 

The Hardikar laboratory is located at The O’Brien Institute, St. Vincents’ Hospital campus in Fitzroy, Melbourne, Australia and is affiliated to The University of Melbourne, Melbourne, Australia.  For an area map of OBI, Melbourne, please click here.  The Diabetes and Stem Cells Section is located at National Center for Cell Science, an autonomous institute of the Department of Biotechnology, Government of India.  The center is located in the campus of University of Pune, Pune, India.  For an area map of NCCS, Pune, click here.

 

 

 

 

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Last revised: June 23, 2010